• Activity And Mechanism Of Action Of Way-150138 Against Herpes Simplex Virus Type 2

      LINDSEY, PHILLIP TY
      Resistance to currently approved drugs and a medical need to treat beta and gamma herpesviruses merit the identification and development of alternative drug therapies for herpesviral infections. Previous research has suggested that a novel class of small molecule thiourea analogs target the UL6 portal protein of HSV-1 and HSV-2, disrupting the replication cycle during the viral DNA encapsidation process. Specifically, WAY-150138 compound was shown to have notable inhibitory effect against HSV-1. The compound’s reported lack of efficacy against HSV-2 remained puzzling considering the high amount of genetic conservation shared in the portal protein between HSV-1 and HSV-2. The studies presented in this thesis sought to resolve this issue. Viral yield assays were conducted for multiple strains of HSV-1 and HSV-2 in the presence of compound, and viral titers were calculated via direct plaque assay on Vero cell monolayers. Contrary to previous studies, WAY-150138 exhibited a similar inhibitory effect against both HSV-1 and HSV-2. To further investigate the molecular mechanism of thiourea compounds that target encapsidation, the HSV-2 portal homolog, encoded by the UL6 gene, was used to generate an rb-BAC-pUL6 protein. After full-length expression of the portal protein in a recombinant baculovirus system, sucrose gradient fractionation provided samples containing enriched amounts of pUL6 for further biochemical analysis.
    • Adult Siblings Of Individuals With Developmental And Psychiatric Disabilities: Relationship Quality, Coping, And Plans For The Future

      Rice, Margarita Velez
      The following study examines the experience of adult siblings of individuals who have developmental and psychiatric disabilities. Using a mixed methods research design, the researcher evaluated 84 self-reports of adult sibling relationship quality, coping strategies, and responses to qualitative questions regarding relational characterizations and expectations for the future planning and care for brothers and sisters with special needs. This study identified significant correlations between warm sibling relationships and coping strategies. A thematic analysis of the qualitative responses revealed themes that created complementarity to the quantitative findings and indicate directions for future research.
    • Aesthetics, The Purpose Of Education, And The Process Of Schooling / By Claire Amy Schultz.

      Schultz, Claire Amy
      Abstract Claire Amy Schultz Aesthetics, the purpose of education, and the process of schooling Under the direction of Vincent W. Youngbauer, Ph.D. In the American education system, approaches in schooling methods are closely tied to pragmatic and positivistic theoretical paradigms, relying on universalized and generalized curricula, where the performance of an individual is displayed in quantifiable terms. High-stakes testing and other quantitative performance measures limit pedagogical practices and curriculum theory, resulting in the economy managing curricula. A purpose of this qualitative research project is to initiate and contribute to a conversation about the purpose of education and the process of schooling. This study developed a theoretical framework with aesthetic and antiaesthetic lenses to examine students’ perceptions of schooling and education. The process of schooling and the purpose of education are discussed in this research through a hermeneutical heuristic in the context of the developed theoretical framework of aesthetics. Data were collected using an open-ended survey instrument. Follow-up interviews with participants helped to clarify some of the responses from the initial survey data. The results of this research indicated that the student participants conflate schooling and education. Economic concerns were most prevalent in the data collected, suggesting that the purpose of education and the process of schooling are for the narrow purpose of obtaining employment. This research examines this link and offers insight to shifting the curriculum theories that drive the current trends of pragmatism and positivism in education toward an aesthetic viewpoint.
    • African American Clergy Engaging In Pastoral Care And Counseling And Affirmative Counseling With Sexual Minorities

      Estelhomme, Cherry D.
      This qualitative research study will look at how African American Pastors and clergy members offer effective Pastoral Care and Counseling and Affirmative Counseling regardless of their theological understanding of homosexuality and same-sex marriage, in the context of Biblical interpretation, morality, and civil rights. In the African American community, clergy members are frequently viewed as the binding agent that holds all things together, including influencing family stability and areas of social concern. These clergy members have been tasked with counseling parishioners who are dealing with some of the most complex questions and patterns of thought, including political issues of social, racial, and economic equality. African American clergy members tend to preach scriptural texts from a literal point of view especially regarding sexual morality and have traditionally been strongly opposed to same sex marriage (Robertson, & Avent, 2016). These same ethnic minorities were accused of perpetrating the same discrimination that has held down their own disadvantaged racial communities. (Brown, 2007). However, for many of these clergy members, the debate has not always been about civil and/or legal rights, instead the argument against same-sex marriage had more to do with Biblical interpretation and moral standings (Barnes, 2013; Waweru, 2009). Keywords: African American, clergy, Pastor, The Black Church, marriage, same-sex marriage, Pastoral Care and Counseling, Affirmative Counseling/Therapy, interracial marriage, homosexual, homosexuality, homophobic, heterosexual, heteronormative, heterosexist, gender, pastoral care, race, religion, theology, Biblical interpretation, morality, civil rights, race relations.
    • African American Millennial College Students' Appraisal Of Failed Attempts At Pursuing Student Leadership Opportunities / By Michael Gary.

      Gary, Michael
      Millennials college students report more mental health problems. Research about Millennial college students often neglects the experiences of Millennials college students of color. African American Millennial college students’ experience mental health issues as their third major cause of death by suicide, with the leading predictor being depression. The study explored the lived experiences and coping responses of African American Millennial college students who have faced failed attempts at obtaining student leadership positions, as well as the appraisal processes students utilize while going through this experience. The researcher employed a qualitative method to explore this phenomenon by conducting a thematic analysis using data collected via a semistructured interview questionnaire to determine (a) In what way do African American Millennial college students appraise the lived experience of a failed attempt at a student leadership position? and (b) How do African American Millennial college students at one Historically Black College or University describe their coping responses to a failed attempt at a pursuing a student leadership position? 1. The researcher inquired about Millennials' (a) Character and Desire to Achieve; (b) Experience and Reaction to Not Being Selected; (c) Reaction about Process and Purpose for Student Leadership Roles; (d) Personal Retrospect: Ability to Cope and Express Oneself; (e) Reaction/Response to Loss or Failure; (d) Actualization and Self-Evaluation; and (f) Emotional Support and Closure. The themes that emerged were: (a) Passionate, Outgoing, and Persistent; (b) Disappointed and Self-Deprecating; (c) Desire to Advocate, Inspire, and Influence; (d) Feels Defeated, Doubtful, Inadequate, and Discouraged; (e) Copes Reclusively in Order to Refocus and Move-On; (f) Self-Actualization Leads to Evaluation, Expression, and Blame; and (g) Lacks Emotional Support and Desires Closure. The students were passionate about pursing leadership positions, yet ended up with feelings of anger, hurt, disappointment, incapability, and sadness. Nonetheless, the students did not convey behaviors of entitlement. Participants reported working to resolve feelings of depression and anxiety by withdrawing from campus life (avoidance coping) to gain perspective regarding their failed attempt, which suggests anxiety and depression are prominent among African American Millennial college students.
    • Am I Enough?: A Mixed-Methods Investigation of the Academic Influences of Black Women in Counselor Education and Supervision Doctoral Programs

      Williams-Whitley, Ashley Nicole; College of Professional Advancement
      Despite the high retention rate and challenges of program preparation, counselor education programs are inevitably challenging previous approaches to ensure that students are staying in their programs (Denecke et al., 2017; Evans et al., 2018; Zhou & Okahana, 2016). The purpose of this study is to establish a foundation by identifying the factors that lead to academic achievement among Black women in counselor education and supervision programs. This study used a convergent mixed-methods approach using a One-Way ANOVA and Grounded Theory to identify and examine emotional exhaustion, microaggressions, peer mentorship, and supportive networks as academic influences that affect the retention and matriculation process of Black Women in Counselor Education and Supervision doctoral programs. The theoretical framework used Brooks’ (1994) Critical Race Theory (as used in the educational context) and Collin’s (1989) Black Feminist Theory to understand experiences and outcomes through the lens of Black women. Results, implications, and future research were discussed
    • An Analysis of the Toxic Effects of Mercury Cyanide Complexes on Zebrafish

      Pittman, Elizabeth; School of Medicine
      Mercury (Hg) is a unique heavy metal toxicant that is found in numerous environmental and occupational settings. A major source of environmental Hg is from artisanal and small-scale gold mining (ASGM), whereby metallic mercury (Hg0) is used to amalgamate gold from mined ore. Hg extraction of gold is inefficient and thus, a significant amount of gold remains in the ore. The leftover Hg-contaminated tailings are often subjected to cyanidation to extract the remaining gold. During this process, mercury and cyanide form mercuric cyanide complexes that are held together with a strong, covalent Hg-carbon bond. These toxic complexes are proposed to be prevalent in terrestrial and aquatic environments around mining sites. The purpose of the current study was to determine how mercuric cyanide complexes, particularly Hg(CN)2, affect the health of aquatic organisms in contaminated environments. We used adult and larval zebrafish (Danio rerio) as model organisms for this study. Zebrafish, in various life stages, were exposed to several different concentrations of Hg(CN)2 to determine the physical and behavioral effects of this exposure. Embryos were exposed to varying concentrations of Hg(CN)2 at one-hour post fertilization (4th cell cycle) until 72 hours post fertilization when hatching begins. Concentrations ranged from 0 to 0.1 mg/L Hg(CN)2. Embryos exposed to concentrations of Hg(CN)2 above 0.08 mg/L had a lower hatching rate and survival rate. Exposure of adult fish to various concentrations of Hg(CN)2 led to significant alterations in behavior and mercury content of muscle. The current study is the first to report the way in which Hg(CN)2 affects aquatic organisms in various stages of life.
    • An Analysis Of Title Ix Compliance In Historically Black Colleges And Universities

      Coursey, Caroline Coker
      Historically black colleges and universities (HBCUs) generally have majority female student populations, yet some studies have concluded that HBCUs have poorer compliance with Title IX when compared with predominantly white institutions (Ruckman & Bridges, 2014; Anderson et al, 2006). Therefore, this study analyzed Title IX compliance in a group of HBCUs in 2003, 2010, and 2016 to determine trends in compliance by computing a proportionality gap percentage for each selected year. A quantitative research design was utilized, and enrollment data was analyzed via an ANOVA method to determine if statistically significant changes in enrollment occurred at the 2003, 2010, and 2016 years. The enrollment data was compared to the proportionality gap percentages for 2003, 2010, and 2016 via Pearson correlation to determine if a relationship exists between enrollment and compliance. Lastly, endowment data was analyzed for a subset of institutions to determine if any relationship exists between endowment size and compliance with Title IX.